Operational data for the risk management of victim operated explosive devices in humanitarian mine action: A Practitioner's perspective.

Since Mine Action's inception at the end of the 1980s, operators have collected limited data on the Victim Operated Explosive Devices (VOEDs) they clear. This includes not only data on the explosive ordnance itself but data on how they were found, where they were found and how they were processed and ultimately destroyed. In a context where detection of mines, boobytraps and certain Victim Operated Improvised Explosive Devices (VOIEDs) is an ongoing humanitarian and military challenge, significantly expanded operational data collection provides an achievable way to facilitate enhanced operational risk management. Risk decisions inherent in the clearance of VOEDs are better if made on the basis of extensive operational data. In the absence of a technological solution to detect and positively discriminate VOEDs from false positive indications, the collection of operational data offers the best prospect for "managing" if not "solving" the problem.

Silica Nanoparticle/Fluorescent Dye Assembly Capable of Ultrasensitively Detecting Airborne Triacetone Triperoxide: Proof-of-Concept Detection of Improvised Explosive Devices in the Workroom.

We describe the proof of concept of a portable testing setup for the detection of triacetone triperoxide (TATP), a common component in improvised explosive devices. The system allows for field testing and generation of real-time results to test for TATP vapor traces in air by simply using circulation of the air samples through the sensing mechanism under the air conditioning system of an ordinary room. In this way, the controlled trapping of the analyte in the chemical sensor gives reliable results at extremely low concentrations of TATP in air under real-life conditions, suitable for daily use in luggage storage for airlines or a locker room for a major sporting event. The reported fluorescent methodology is very sensitive and selective, allowing for the trapping of triacetone triperoxide in the chemical sensor to give reliable results at very low concentrations in air under ambient conditions, by comparing the fluorescence of the material before and after exposition to TATP traces in air.

Determination of triacetone triperoxide (TATP) traces using passive samplers in combination with GC-MS and GC-PCI-MS/MS methods.

The use of organic peroxides for the preparation of homemade explosives (HMEs) is common among terrorists due to inexpensive precursor chemicals and simple synthetic procedures. Triacetone triperoxide (TATP) is the most notable peroxide explosive, and has been deployed in several terrorist attacks as explosive filling of improvised explosive devices (IEDs). Forensic identification of TATP in pre-blast and post-blast residues, including on-site analysis, poses significant analytical challenges and induces demand for practicable and sensitive detection techniques. This work presents a concept suitable for laboratory and on-site identification of TATP residues in liquid samples (aqueous TATP synthetic waste) and in gas phase. It is based on TATP enrichment from the aqueous or gas phase using different types of passive samplers (polydimethylsiloxane (PDMS) sampling rods and activated carbon sampling tubes (ACST)) and subsequent identification of the explosive by gas chromatography-mass spectrometry (GC-MS) or GC with positive chemical ionization and tandem MS (GC-PCI-MS/MS) analytical techniques. Additionally, investigation of the stability of TATP in aqueous solutions and of the stability of enriched TATP in passive samplers under different storage conditions, as well as development of TATP re-extraction procedures from passive samplers have been performed in this study. The practical use of passive samplers was demonstrated during and after TATP production processes. Moreover, post-blast sampling of TATP under different conditions of controlled blasting events was investigated using the passive sampling concept.

Implementation of and Experimentation with Ground-Penetrating Radar for Real-Time Automatic Detection of Buried Improvised Explosive Devices.

This paper proposes the implementation of and experimentation with GPR for real-time automatic detection of buried IEDs. GPR, consisting of hardware and software, was implemented. A UWB antenna was designed and implemented, particularly for the operation of the GPR. The experiments were conducted in order to demonstrate the real-time automatic detection of buried IEDs using GPR with an R-CNN algorithm. In the experiments, the GPR was mounted on a pickup truck and a maintenance train in order to find the IEDs buried under a road and a railway, respectively. B-scan images were collected using the implemented GPR. R-CNN-based detection for the hyperbolic pattern, which indicates the buried IED, was performed along with pre-processing, for example, using zero offset removal, and background removal and filtering. Experimental results in terms of detecting the hyperbolic pattern in B-scan images were shown and verified that the proposed GPR system is superior to the conventional one using region analysis processing-based detection. Results also showed that pre-processing is required in order to improve and/or clean the hyperbolic pattern before detection. The GPR can automatically detect IEDs buried under roads and railways in real time by detecting the hyperbolic pattern appearing in the collected B-scan image.

Perfil neuropsicológico de un grupo de militares heridos por artefacto explosivo / Neuropsychological profile of a group of military injured by explosive artefact

Resumen. Las heridas por artefactos explosivos y las secuelas causadas por estos se han convertido en el foco de diversos estudios en el ámbito médico y psicológico, aunque en el entorno neuropsicológico son pocos los hallazgos encontrados, debido a esto la presente investigación surge con el fin de describir el perfil neuropsicológico de un grupo de militares colombianos heridos por artefactos explosivos. Participaron 60 militares colombianos heridos y se les realizó un análisis descriptivo de las funciones neuropsicológicas a partir de la batería Neuropsi Atención y Memoria. Los participantes presentaron un déficit de leve a moderado en la curva de memoria de codificación y evocación; memoria verbal espontánea, memoria por claves y en memoria lógica, igualmente, en procesos ejecutivos de organización de la conducta, capacidad de planeación e inhibición de conductas. De igual forma, se encontró una alteración de moderada a severa en atención sostenida, planificación y codificación visual. Los resultados son discutidos con la literatura existente.

Abstract. Injuries from explosive devices and the sequelae caused by them have become the focus of various studies in the medical and psychological fields, although in the neuropsychological environment there are few findings, due to this the present investigation arises in order to describe the neuropsychological profile of a group of Colombian soldiers injured by explosive devices. Sixty wounded Colombian soldiers participated and a descriptive analysis of the neuropsychological functions was carried out from the Neuropsi Attention and Memory battery. The participants had a mild to moderate deficit in the coding and recall memory curve; Spontaneous verbal memory, memory by keys and in logical memory, likewise, in executive processes of behavior organization, planning capacity and behavior inhibition. Similarly, a moderate to severe alteration was found in sustained attention, planning and visual coding. The results are discussed with the existing literature.

Profile of explosives's use in ATMs/cash safes robberies in Brazil.

This retrospective study reports data obtained by the Federal Police's National Institute of Criminalistics (INC-PF) relating to chemical analysis aimed at identifying explosives used in Automated teller machines (ATMs)/cash safes robberies between 2014 and 2020 in Brazil. 93 Real cases were studied and, based on the analysis carried out on the materials related to these cases, focusing on the type of explosive used, the following distribution profile was obtained: I) explosive mixtures based on chlorates and/or perchlorates (53%); explosive emulsion (22%); black gunpowder (13%); negative/inconclusive (11%) and organic - pentaerythritol tetranitrate (PETN) (1%). These results can contribute to investigations related to diversion/loss of explosives for criminal purposes, indicating, for example, through relationships between prevalence in the use of a certain type of explosive with a certain location, its possible origin (mining industry, explosive industries, fireworks factories, among others). The profile observed in the results can guide the selection of explosives to be studied in future research, as the possibilities are vast. Furthermore, despite the expressive number of occurrences in Brazil in the period of interest of this study, only a small fraction of samples was sent to the forensic chemistry laboratory to identify the explosive involved, which suggests that expanding chemical analysis should be encouraged in this field. In combination with an increase in professional training and collaboration trials between the laboratories, these activities can improve the chemical explosive's profile in Brazil, enabling the search for correlations between occurrences and contributing to the growth and development of this area.

Military Dogs and Their Soldier Companions: The More-than-human Biopolitics of Leishmaniasis in Conflict-torn Colombia.

Cutaneous leishmaniasis is a vector-borne disease that produces growing skin ulcers. In Colombia, the transmitting phlebotomine sandfly is native to the same jungles that have been the primary theater of war. Although combatants are the most affected by leishmaniasis, military landmine detection dogs are also significantly impacted. This article draws on ethnographic field research with human and canine members of the Colombian military. While their leishmaniasis ulcers constitute a shared expression of violence that makes evident the closeness of the human-dog bond, differences in their state-provided health care reveal the production of shifting species hierarchies. I argue that war scrambles both human-dog affective relationships and biopolitically configured interspecies hierarchies in ways that produce suffering, not just for humans and dogs separately, but also for the bonds they forge together. Building peace through health care demands repairing the ways in which armed violence has rendered the bonds between humans and nonhumans pathological.

Ocular biomechanics during improvised explosive device blast: A computational study using eye-specific models.

BACKGROUND: Eye injuries comprise 10-13% of civilian improvised explosive device (IED) injuries. The bomb blast wave induces a normal and shear forces on the tissues, causing a large acute IOP elevation. This study calculated the biomechanical stresses and strains in the eye due to IED explosion via eye-specific fluid-structure interaction (FSI) models. METHODS: Blast occurred at 2, 3, and 4 m from the front and side of the victim and the weights of the IED were 1 and 2 kg. The ground was covered with the deformable soil to mimic the realistic IED explosion condition and reflect the blast wave. RESULTS: The IOP elevation of ∼6,000-48,000 mmHg was observed in the eyes while the highest IOP was occurred with the IED weight and distance of 2 kg and 2 m (front) and the lowest was occurred with the IED weight and distance of 1 kg and 4 m (side). Our findings suggest the importance of the victim location and orientation concerning the blast wave when it comes to ocular injury assessment. IOP elevation of ∼2900 and ∼2700 mmHg were observed in ∼1.6 ms after the blast for the IEDS weight of 2 kg and a victim distance of 2 m in front and side blasts, respectively, in consistence with the literature. Nonetheless, IOPs were considerably higher after ∼1.6 ms due to the merging of the bomb blast wave and its reflection off the ground. CONCLUSIONS: The stresses and strains were highest for the frontal blast. Both side and frontal blasts caused higher stresses and strains at the rectus muscle insertions where the sclera is thinnest and prone to rupture. Blast angle has no considerable role in the resultant IOP. Front blast with a heavier IED resulted a higher stresses and deformations in the eye connective tissues compared to the side blast.

Ocular biomechanics due to ground blast reinforcement.

BACKGROUND AND OBJECTIVE: Bomb blast injuries exerts a shearing force on the air-tissue interfaces, causing devastating ocular injury from the blast wave. Improvised explosive devices (IEDs) are usually placed at different heights from the ground to induce more severe injury through ground blast reinforcement (GBR). However, there is still a lack of knowledge of the role of GBR and IED height from the ground on ocular biomechanics, and how they can affect the intraocular pressure (IOP) in the eye. This study aimed to estimate the IOP due to frontal IED explosion at different heights from the ground using a fluid-structure interaction model with and without GBR effects. METHODS: A 2 kg IED was placed within 5 m of the victim at 5 different heights from the ground, including 0, 0.42, 0.85, 1.27, and 1.70 m. Two different blast formulations were used to simulate the IED explosion: (a) spherical airburst, with no amplification of the initial shock wave due to interaction with the ground-surface, and (b) hemispherical surface-burst, where the initial blast wave is immediately reflected and reinforced by the ground (GBR). RESULTS: Results revealed that the blast wave due to GBR reaches to the skull prior to the IED blast itself. The GBR also reached to the skull ∼ 0.6 ms earlier when the IED was on the ground compared to the height of 1.70 m. The highest and lowest IOPs of ∼ 17,000 and ∼ 15,000 mmHg were observed at the IED heights of 1.70 and 0 m from the ground considering GBR. However, when the role of the GBR is ignored, IOP of ∼ 9,000 mmHg was observed regardless of the IED height from the ground. The deformation in the apex of the cornea was higher when considering the GBR (∼ 0.75 cm) versus no GBR (∼ 0.65 cm). Considering GBR led to higher stresses and strains in the sclera. CONCLUSIONS: When the role of GBR was ignored, the results showed similar patterns and magnitudes of stresses and deformations in the skull and eye regardless of the height of the IED from the ground, which was not the case when GBR was considered. The findings of this study suggest the critical role of GBR in ocular blast simulations.

The forensic analysis of homemade explosive suicides: Case report and systematic review of the literature.

The illegal construction of explosive devices for recreational purposes has become increasingly widespread in recent years. This phenomenon is spurred on by numerous websites that explain how to build a self-made device. The correlation between the use of firearms and suicide is known in the literature, but the use of explosive devices and self-harm is little studied. Unfortunately, the risk associated with the manufacture of homemade explosives is poorly known. For this purpose, we describe a rare suicide carried out by a man suffering from depressive disorder with psychotic and delusional features. The forensic investigations at the scene showed that the man had designed a homemade device, and disseminated numerous suicide notes in his home, transcribed in the previous weeks in which he revealed his suicidal motivation. Crime scene investigation showed on the body and on the road in front of the explosion point: multiple fragments of glass (zone 1), a lighter with blood stains (zone 2), and shreds of clothing scattered along the road (zone 3). Autopsy revealed that the cause of death was extensive blunt injury to head and trunk due to barotrauma due to the explosion of a homemade device. The case is compared with data from a systematic review of the forensic literature on suicides involving explosives. The study proposes the analysis of planimetric areas in these crime scene investigations and active surveillance in these subjects.

Raman spectroscopy for detection of ammonium nitrate as an explosive precursor used in improvised explosive devices.

Raman spectroscopy was evaluated as a sensor for detection of ammonium nitrate (NH4NO3, AN), fuel oil (FO), AN-water solutions, and AN- and FO-soil mixtures deposited on materials such as glass, synthetic fabric, cardboard and electrical tape to simulate field conditions of explosives detection. AN is an inorganic oxidizing salt that is commonly used in fertilizers and mining explosives, however, due to its widespread accessibility, AN-based explosives are also utilized for the manufacture of improvised explosive devices (IED). Pure AN crystals were ground to powder size and deposited on several substrates for Raman analysis, whereas FO was analysed in a quartz cuvette. To simulate field conditions samples of powdered AN, AN-water solutions (0.1% to 10.0% AN w/w), AN-soil (50% to 90% AN w/w) and FO-soil (50% to 75% FO w/w) were prepared and deposited on the clutter materials. Raman spectra were acquired at integration times between 0.1 and 30 s, and 3 replicate Raman measurements were carried out for each sample. The spectral window observed ranged from 300 to 3800 cm-1. Several characteristic Raman bands were found, namely, at 710 cm-1 (NO3-) and 1040 cm-1 (NO3-) for AN; 1440-1470 cm-1 (CH) and 2800-3000 cm-1 (CH) for FO; 3000-3500 cm-1 (OH) for water; and 615 cm-1 (CCl), 1254 cm-1 (CH), 1400 cm-1 (CH2) and 1600 cm-1 (aromatic ring) for polyvinyl chloride (PVC, electrical tape). The effect of the AN concentration and integration time on the total and net Raman intensities, relative standard deviation, signal-to-noise ratio and relative limit of detection was evaluated. The relative limit of detection of AN in water was 0.1% (1 mg/g), and absolute limit of detection was 1.0 µg. The optimum integration time (≈10 s) for the Raman sensor to capture the analyte signals was estimated based on the Raman figures of merit as a function of the integration time.

Improved STR profiles from improvised explosive device (IED): fluorescence latent DNA detection and direct PCR.

Bombing accounts for the largest share of terrorist incidents worldwide. Most involve an improvised explosive device (IED): a bomb made from household items. Touch DNA may be left on parts of an IED during assembly. However, an IED conflagration degrades DNA, and there has never been a way to locate where touch DNA may remain. To solve this problem, we combined the use of fluorescent dye to locate latent DNA and direct PCR to improve STR profiles of DNA obtained from IEDs. Six fluorescent DNA-binding dyes were evaluated at various concentrations for the purpose of staining latent DNA. SYBR® Green I and Diamond™ Nucleic Acid dye were able to visualize touch DNA on IED substrates. Inhibition studies with extracted DNA and touch DNA using both dyes revealed that Diamond™ dye inhibited direct STR amplification, while SYBR® Green I did not. Stability studies at three temperatures showed optimum performance of SYBR® Green I up to 24 h after formulation. As such, only SYBR® Green I was further used to develop a "visualized-direct PCR" method. Using the conventional approach and the novel "visualized-direct PCR" approach in a single-blind investigation of mock IED evidence, the "visualized-direct PCR" approach had a 98.6% chance of obtaining more alleles (95% highest density interval (HDI): 0.7 to 10.0 alleles). A decrease in non-donor's alleles (mixed profiles) was also observed. The developed approach has the potential to revolutionize the process of STR typing from touch DNA.

Treatment Strategies in the Management of Maxillofacial Ballistic Injuries in Low-Intensity Conflict Scenarios.

INTRODUCTION: The facial disfigurement and functional debility resulting from craniomaxillofacial injuries in low-intensity conflict scenarios can physically and psychologically traumatize the afflicted personnel. Efficient and definitive management, with complete esthetic restoration and functional rehabilitation, is not only an organizational obligation, but also a tactical necessity to maintain a high state of morale among the troops. There exist two schools of thought on principles of management of such injuries. The older, three-phased approach consists of initial debridement and suturing, followed by conservative closed reduction in maxillofacial fractures using splints and ligatures, thereafter followed by delayed repair and late reconstruction of residual bone defects and deformities after the soft tissue healing is complete. The newer trend involves early and aggressive open surgical reduction and craniomaxillofacial fixation techniques along with reconstructive procedures carried out hand in hand with the soft tissue debridement and closure. AIM: The aim was to compare the efficacy of the two management protocols, namely the contemporary approach of early aggressive surgical intervention, versus the conservative approach of initial debridement, closed reduction and delayed repair, as the definitive treatment modality of maxillofacial injuries sustained in low-intensity conflicts. METHODS: This retrospective analytical study included 40 patients with maxillofacial injuries sustained in combat scenarios treated over a period of 3 years. These patients who had been treated for ballistic maxillofacial injuries were divided into two groups: The first group of 20 patients (Group 1) included those who had undergone an early, aggressive, surgical intervention, and the second group of 20 patients (Group 2) included those who had undergone resuscitation and primary soft tissue closure followed by conservative, closed reduction techniques, delayed repair (including open reduction and internal fixation (ORIF) procedures), and late reconstruction of bone soft tissue defects (which included utilization of various grafts and flaps). Both groups were evaluated and compared for postoperative recovery and early and late complications such as impaired esthetic results and impaired functional recovery. RESULTS: Early, definitive, and aggressive maxillofacial surgical techniques proved superior to the conservative approach by bringing about primary bone healing and minimizing residual deformities and subsequent scar contractures, thus yielding improved functional as well as superior esthetic outcomes. CONCLUSION: In today's low-intensity conflict scenario, the emphasis in management of maxillofacial injury victims should be on an early, definitive, and aggressive surgical repair and reconstruction of the facial skeleton, thus restoring quality of life to these soldiers, sparing them life-long indignity after a potentially severe esthetically and functionally debilitating injury.

Risk factors for unsafe behaviors toward grenades among rural populations affected by explosive devices in Colombia.

BACKGROUND: Following decades of armed conflict, Colombia remains highly affected by explosive device (ED) contamination, especially in rural areas. Many victims are injured by EDs despite knowing their dangers. Determining risk factors for unsafe behaviors toward EDs, including grenades, is critical for preventing injuries. METHODS: In 2012, CDC assisted Colombian partners in conducting a multi-stage knowledge, attitudes, and practices survey in rural ED-affected areas. Within each of 40 clusters, 28 households were selected, and participants aged 10 years or older were asked about behaviors toward EDs. Participants reported actual behaviors toward past EDs encountered and theoretical behaviors toward EDs not encountered. Behaviors were a priori classified as unsafe or safe. This analysis focuses on behaviors toward the most commonly encountered device, grenades. RESULTS: Of 928 adult and 562 child participants, 488 (52.5%) adults and 249 (43.9%) children encountered ED, while 121 (13.7%) adults and 148 (26.9%) children received mine risk education (MRE). Among the 430 (46.7%) adults who encountered grenades, 113 (25.7%) reported unsafe behaviors; multivariable analysis showed that unsafe behavior was associated with working outdoors (adjusted odds ratio [aOR]: 1.7, 95% confidence interval [CI]: 1.1-2.7). Among the 429 (46.5%) adults who did not encounter ED, 61 (14.6%) described unsafe theoretical behaviors toward grenades; multivariable analysis showed that unsafe behavior was associated with older age (aOR: 1.02, 95% confidence limit [CL]: 1.00-1.05) and black or Afro-Colombian identity (aOR: 2.5, 95% CI 1.3-5.1). Among the 181 (32.0%) children who encountered grenades, 41 (23.8%) reported unsafe behaviors, while among the 311 (55.9%) children who did not encounter ED, 30 (10.2%) reported unsafe behavior. In both groups of children, multivariable analysis showed that unsafe behavior was associated with lower mean score on knowledge of ED, with aOR: 0.7, 95% CL: 0.6-0.9, and aOR: 0.8, 95% CL: 0.6-0.98, respectively. CONCLUSIONS: Participants reported frequent ED exposure but low receipt of MRE. Our findings should guide MRE improvement in ED-affected areas by strengthening the connection between ED knowledge and avoiding unsafe behavior, with a particular focus on people working outdoors. MRE should promote knowledge of ED risks but should also recognize socioeconomic factors that lead to engaging in unsafe behaviors.

Characteristics of mandibular injuries caused by bullets and improvised explosive devices: a comparative study.

The purpose of this study was to investigate the difference in mandibular trauma caused by two mechanisms for the delivery of missile injuries: firearms and improvised explosive devices (IEDs). The data investigated included sex, age, mechanism of injury, and other clinical and radiographic manifestations. Seventy consecutive patients, predominantly male, with a mean age of 28.6±14 years (range 2-60 years) were enrolled: 38 patients (54.3%) sustained mandibular fractures caused by bullet injuries and 32 patients (45.7%) had mandibular fractures caused by IED explosion injuries. The study revealed that the differences in most of the investigated variables were not statistically significant; the only significant differences were the incidence of trauma to other body regions and the presence of retained foreign bodies. The effect on the mandible of IED explosion resembles that of bullets. Bullet injuries are associated with a relatively higher number of mandibular sites involved and more bilateral fractures, in addition to more extensive bone loss. IEDs, on the other hand, cause more multisystem injuries and result in more retained foreign bodies.

An experimental study addressing the use of geoforensic analysis for the exploitation of improvised explosive devices (IEDs).

The use of geoforensic analysis in criminal investigations is continuing to develop, with the diversification of analytical techniques, many of which are semi-automated, facilitating prompt analysis of large sample sets at a relatively low cost. Whilst micro-scale geoforensic analysis has been shown to assist criminal investigations including homicide (Concheri et al., 2011 [1]), wildlife crime (Morgan et al., 2006 [2]), illicit drug distribution (Stanley, 1992 [3]), and burglary (Mildenhall, 2006 [4]), its application to the pressing international security threat posed by Improvised Explosive Devices (IEDs) is yet to be considered. This experimental study simulated an IED supply chain from the sourcing of raw materials through to device emplacement. Mineralogy, quartz grain surface texture analysis (QGSTA) and particle size analysis (PSA) were used to assess whether environmental materials were transferred and subsequently persisted on the different components of three pressure plate IEDs. The research also addressed whether these samples were comprised of material from single or multiple geographical provenances that represented supply chain activity nodes. The simulation demonstrated that material derived from multiple activity nodes, was transferred and persisted on device components. The results from the mineralogy and QGSTA illustrated the value these techniques offer for the analysis of mixed provenance samples. The results from the PSA, which produces a bulk signature of the sample, failed to distinguish multiple provenances. The study also considered how the environmental material recovered could be used to generate information regarding the geographical locations the device had been in contact with, in an intelligence style investigation, and demonstrated that geoforensic analysis has the potential to be of value to international counter-IED efforts. It is a tool that may be used to prevent the distribution of large quantities of devices, by aiding the identification of the geographical location of key activity nodes.

Acetone facilitated DNA sampling from electrical tapes improves DNA recovery and enables latent fingerprints development.

Electrical tapes (ETs) are a common component of improvised explosive devices (IEDs) used by terrorists or criminal organizations and represent a valuable forensic resource for DNA and latent fingerprints recovery. However, DNA recovery rates are typically low and usually below the minimal amount required for amplification. In addition, most DNA extraction methods are destructive and do not allow further latent fingerprints development. In the present study a cell culture based touch DNA model was used to demonstrate a two-step acetone-water DNA recovery protocol from ETs. This protocol involves only the adhesive side of the ET and increases DNA recovery rates by up to 70%. In addition, we demonstrated partially successful latent fingerprints development from the non-sticky side of the ETs. Taken together, this protocol maximizes the forensic examination of ETs and is recommended for routine casework processing.

Analysis of DNA from post-blast pipe bomb fragments for identification and determination of ancestry.

Improvised explosive devices (IEDs) such as pipe bombs are weapons used to detrimentally affect people and communities. A readily accessible brand of exploding targets called Tannerite® has been identified as a potential material for abuse as an explosive in pipe bombs. The ability to recover and genotype DNA from such weapons may be vital in the effort to identify suspects associated with these devices. While it is possible to recover DNA from post-blast fragments using short tandem repeat markers (STRs), genotyping success can be negatively affected by low quantities of DNA, degradation, and/or PCR inhibitors. Alternative markers such as insertion/null (INNULs) and single nucleotide polymorphisms (SNPs) are bi-allelic genetic markers that are shorter genomic targets than STRs for amplification, which are more likely to resist degradation. In this study, we constructed pipe bombs that were spiked with known amounts of biological material to: 1) recover "touch" DNA from the surface of the device, and 2) recover traces of blood from the ends of wires (simulated finger prick). The bombs were detonated with the binary explosive Tannerite® using double-base smokeless powder to initiate the reaction. DNA extracted from the post-blast fragments was quantified with the Quantifiler® Trio DNA Quantification Kit. STR analysis was conducted using the GlobalFiler® Amplification Kit, INNULs were amplified using an early-access version of the InnoTyper™ 21 Kit, and SNP analysis via massively parallel sequencing (MPS) was performed using the HID-Ion Ampliseq™ Identity and Ancestry panels using the Ion Chef and Ion PGM sequencing system. The results of this study showed that INNUL markers resulted in the most complete genetic profiles when compared to STR and SNP profiles. The random match probabilities calculated for samples using INNULs were lower than with STRs when less than 14 STR alleles were reported. These results suggest that INNUL analysis may be well suited for low-template and/or degraded DNA samples, and may be used to supplement incomplete or failed STR analysis. Human identification using SNP analysis via MPS showed variable success with low-level post-blast samples in this study (<150pg). While neat DNA samples (6µL input as recommended) resulted in <50% of SNP calls, samples that were concentrated from 15µL to 6µL (15µL was added for STR and INNUL typing) resulted in more complete SNP profiles. Five out of six blood samples recovered from the wires attached to the pipe-bombs resulted in the correct ancestry predictions.

Identification of the Emplacement of Improvised Explosive Devices by Experienced Mission Payload Operators.

Improvised Explosive Devices (IEDs) have become one of the deadliest threats to military personnel, resulting in over 50% of American combat casualties in Iraq and Afghanistan. Identification of IED emplacement is conducted by mission payload operators (MPOs). Yet, experienced MPOs are limited in number, making MPO training a critical intervention. In this article, we implement a Cognitive Engineering Based on Expert Skill methodology to better understand how experienced MPOs identify the emplacement of IEDs for the purposes of improving training. First, expert knowledge was elicited through interviews and questionnaires to identify the types of perceptual cues used and how these cues are cognitively processed. Results indicate that there are many different static and dynamic cues that interact with each other over time and space. Using data from the interviews and questionnaires, an empirically grounded framework is presented that explains the cognitive process of IED emplacement detection. Using the overall findings and the framework, IED emplacement training scenarios were developed and built into a simulation.